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phenol red free opti mem tm i reduced serum medium  (Thermo Fisher)


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    Structured Review

    Thermo Fisher phenol red free opti mem tm i reduced serum medium
    Phenol Red Free Opti Mem Tm I Reduced Serum Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/opti+mem+i+reduced+serum+medium/Phenol+Red/bio_rxiv__64898__2026__04__10__717554-212-58-66
    Average 99 stars, based on 1 article reviews
    phenol red free opti mem tm i reduced serum medium - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Transfection:

    Article Title: CRISPRi-based screen of Autism Spectrum Disorder risk genes in microglia uncovers roles of ADNP in microglia endocytosis and uptake of synaptic material
    Article Snippet: .. This mixture was diluted with Opti-MEM I Reduced Serum Medium (Gibco; Cat. No. 31985070) before adding Lipofectamine 2000 Transfection Reagent (Invitrogen; Cat. No. 11668027). ..

    Article Title: Free fatty acids induce bile acids overproduction and oxidative damage of bovine hepatocytes via inhibiting FXR/SHP signaling.
    Article Snippet: Hepatic oxidative injury induced by free fatty acids (FFA) and metabolic disorders of bile acids (BA) increase the risk of metabolic diseases in dairy cows during perinatal period.. However, the effects of FFA on BA metabolism remained poorly understood.. In present study, high concentrations of FFA caused cell impairment, oxidative stress and BA overproduction.

    Article Title: Detection and quantification of Na,K-ATPase dimers in the plasma membrane of living cells by FRET-FCS.
    Article Snippet: The sodium potassium pump, Na,K-ATPase (NKA), is an integral plasma membrane protein, expressed in all eukaryotic cells.. It is responsible for maintaining the transmembrane Na + gradient and is the major determinant of the membrane potential.. Self-interaction and oligomerization of NKA in cell membranes has been proposed and discussed but is still an open question.

    Article Title: Superior Induced Pluripotent Stem Cell Generation through Phactr3-Driven Mechanomodulation of Both Early and Late Phases of Cell Reprogramming
    Article Snippet: .. Small interfering RNA (siRNA) against protein phosphatase and actin regulator 3 (Phactr3) or mock scramble siRNA at 4 nM was prepared in Opti-MEM I Reduced Serum Medium (51985-026, Life Technologies) with 16 μl Lipofectamine RNAiMAX Transfection Reagent (13778075, Life Technologies)/ml of solution and incubating the solution at room temperature for 20 min. Then, 1 ml of this solution was mixed with 3 ml of MEF culture medium, and on d1 of routine culture of MEF, at 40% to 50% confluency in T25 flasks, cells were incubated with 4 ml containing siRNA against Phactr3 [ON-TARGETplus mouse Phactr3 (74189) siRNA—SMARTpool, lot-046898-01-0005, Dharmacon) or mock scramble siRNA (DHA-D-001810-10-05 ON-TARGETplus nontargeting pool) at 2 nM and cultured for another 2 day before using them in reprogramming experiments. ..

    Article Title: Trivalent mRNA vaccine-candidate against seasonal flu with cross-specific humoral immune response
    Article Snippet: .. The next day the medium was replaced with a fresh DMEM without antibiotics and cells were transfected by HA-mRNA using Lipofectamine 3000 reagent (Invitrogen) and Opti-Mem I Reduced Serum Medium (Gibco) in accordance with the manufacturer’s instructions. ..

    Article Title: Top-down Proteomics for the Characterization and Quantification of Calreticulin Arginylation
    Article Snippet: When cells reached approximately 70% confluency, each plate was transfected with a total of 2 μg of plasmids using FuGENE® HD (Promega). .. Briefly, plasmids and transfection reagent were premixed in 200 μL of OPTI-MEM I Reduced Serum Medium (Invitrogen), incubated at room temperature for 30 min, and then 800 μL of OPTI-MEM medium was added before dropwise addition to the cells. ..

    Negative Control:

    Article Title: FGF signaling modulates mechanotransduction/WNT signaling in progenitors during tooth root development.
    Article Snippet: .. AllStars Negative Control siRNA (QIAGEN,1027280), Etv5 siRNA (QIAGEN, 1027416, Mm_Etv5_1 FlexiTube siRNA SI00996744; Mm_Etv5_2 FlexiTube siRNA SI00996737; Mm_Etv5_3 FlexiTube siRNA SI00996730; Mm_Etv5_4 FlexiTube siRNA SI00996723), Piezo2 siRNA (QIAGEN, 1027416, Mm_Piezo2_1 FlexiTube siRNA SI04723404; Mm_Piezo2_2 FlexiTube siRNA SI04723397; Mm_Piezo2_3 FlexiTube siRNA SI04723390; Mm_Piezo2_4 FlexiTube siRNA SI04723383), Fzd6 siRNA (QIAGEN, 1027416, Mm_Fzd6_1 FlexiTube siRNA SI02708510; Mm_Fzd6_2 FlexiTube siRNA SI02686684; Mm_Fzd6_3 FlexiTube siRNA SI02666979; Mm_Fzd6_4 FlexiTube siRNA SI00171451), Opti-MEM I Reduced Serum Medium (Thermo Bone Research (2024) 12:37 Fisher, 31985062) and lipofectamineTM RNAiMAX (Thermo Fisher, 13778075) were used in this study. ..

    Over Expression:

    Article Title: Free fatty acids induce bile acids overproduction and oxidative damage of bovine hepatocytes via inhibiting FXR/SHP signaling.
    Article Snippet: Hepatic oxidative injury induced by free fatty acids (FFA) and metabolic disorders of bile acids (BA) increase the risk of metabolic diseases in dairy cows during perinatal period.. However, the effects of FFA on BA metabolism remained poorly understood.. In present study, high concentrations of FFA caused cell impairment, oxidative stress and BA overproduction.

    Incubation:

    Article Title: Detection and quantification of Na,K-ATPase dimers in the plasma membrane of living cells by FRET-FCS.
    Article Snippet: The sodium potassium pump, Na,K-ATPase (NKA), is an integral plasma membrane protein, expressed in all eukaryotic cells.. It is responsible for maintaining the transmembrane Na + gradient and is the major determinant of the membrane potential.. Self-interaction and oligomerization of NKA in cell membranes has been proposed and discussed but is still an open question.

    Article Title: Superior Induced Pluripotent Stem Cell Generation through Phactr3-Driven Mechanomodulation of Both Early and Late Phases of Cell Reprogramming
    Article Snippet: .. Small interfering RNA (siRNA) against protein phosphatase and actin regulator 3 (Phactr3) or mock scramble siRNA at 4 nM was prepared in Opti-MEM I Reduced Serum Medium (51985-026, Life Technologies) with 16 μl Lipofectamine RNAiMAX Transfection Reagent (13778075, Life Technologies)/ml of solution and incubating the solution at room temperature for 20 min. Then, 1 ml of this solution was mixed with 3 ml of MEF culture medium, and on d1 of routine culture of MEF, at 40% to 50% confluency in T25 flasks, cells were incubated with 4 ml containing siRNA against Phactr3 [ON-TARGETplus mouse Phactr3 (74189) siRNA—SMARTpool, lot-046898-01-0005, Dharmacon) or mock scramble siRNA (DHA-D-001810-10-05 ON-TARGETplus nontargeting pool) at 2 nM and cultured for another 2 day before using them in reprogramming experiments. ..

    Article Title: Top-down Proteomics for the Characterization and Quantification of Calreticulin Arginylation
    Article Snippet: When cells reached approximately 70% confluency, each plate was transfected with a total of 2 μg of plasmids using FuGENE® HD (Promega). .. Briefly, plasmids and transfection reagent were premixed in 200 μL of OPTI-MEM I Reduced Serum Medium (Invitrogen), incubated at room temperature for 30 min, and then 800 μL of OPTI-MEM medium was added before dropwise addition to the cells. ..

    Small Interfering RNA:

    Article Title: Superior Induced Pluripotent Stem Cell Generation through Phactr3-Driven Mechanomodulation of Both Early and Late Phases of Cell Reprogramming
    Article Snippet: .. Small interfering RNA (siRNA) against protein phosphatase and actin regulator 3 (Phactr3) or mock scramble siRNA at 4 nM was prepared in Opti-MEM I Reduced Serum Medium (51985-026, Life Technologies) with 16 μl Lipofectamine RNAiMAX Transfection Reagent (13778075, Life Technologies)/ml of solution and incubating the solution at room temperature for 20 min. Then, 1 ml of this solution was mixed with 3 ml of MEF culture medium, and on d1 of routine culture of MEF, at 40% to 50% confluency in T25 flasks, cells were incubated with 4 ml containing siRNA against Phactr3 [ON-TARGETplus mouse Phactr3 (74189) siRNA—SMARTpool, lot-046898-01-0005, Dharmacon) or mock scramble siRNA (DHA-D-001810-10-05 ON-TARGETplus nontargeting pool) at 2 nM and cultured for another 2 day before using them in reprogramming experiments. ..

    Cell Culture:

    Article Title: Superior Induced Pluripotent Stem Cell Generation through Phactr3-Driven Mechanomodulation of Both Early and Late Phases of Cell Reprogramming
    Article Snippet: .. Small interfering RNA (siRNA) against protein phosphatase and actin regulator 3 (Phactr3) or mock scramble siRNA at 4 nM was prepared in Opti-MEM I Reduced Serum Medium (51985-026, Life Technologies) with 16 μl Lipofectamine RNAiMAX Transfection Reagent (13778075, Life Technologies)/ml of solution and incubating the solution at room temperature for 20 min. Then, 1 ml of this solution was mixed with 3 ml of MEF culture medium, and on d1 of routine culture of MEF, at 40% to 50% confluency in T25 flasks, cells were incubated with 4 ml containing siRNA against Phactr3 [ON-TARGETplus mouse Phactr3 (74189) siRNA—SMARTpool, lot-046898-01-0005, Dharmacon) or mock scramble siRNA (DHA-D-001810-10-05 ON-TARGETplus nontargeting pool) at 2 nM and cultured for another 2 day before using them in reprogramming experiments. ..



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